000			023190000a22005890004500
999			_c87969 _d87969
003			CR-TuBCO
005			20221209062720.0
007			ta
008			220328b ||||| |||| 00| 0 eng d
040			_aCR-TuBCO _cCR-TuBCO _bEs
041	0		_aeng
082	0	4	_a633.7314 _bC957
100	1		_963847 _aDussert, S.
100	1		_956148 _aChabrillange, N.
100	1		_9109 _aEngelmann, F.
100	1		_943095 _aAnthony, F.
100	1		_975866 _aHamon, S.
245	1	0	_aCryopreservation on coffee (Coffea arabica L.) seeds: importance of the precooling temperature
260			_c1997
270			_aSan José, C.R.
500			_a26 ref.
520			_aThe effect of the precooling temperature on the survival rate of cryopreserved Coffea arabica seeds and excised zygotic embryos was investigated. Seeds were desiccated to 0.2 g H2O.g-1 dw, then cooled at 1°C.min-1 to various precooling temperatures. The optimal germination rate (70 percent) of cryopreserved seeds was achieved after precooling to -50°C. After 4 months in culture, 30 percent of seeds developed into normal seedlings. For embryos extracted after thawing from cryopreserved seeds, maximal survival rate (97 percent) was observed when seeds were immersed into liquid nitrogen directly after dehydration, without precooling. All viable embryos developed into normal seedlings after 2 months in culture.
650	1	4	_9140993 _aCOFFEA ARABICA
650	1	0	_aSEMILLAS _9133847
650	1	4	_9145969 _aENFRIAMIENTO
650	1	4	_9144137 _aDESHIDRATACION
650	1	4	_98 _aCONSERVACION DE LOS RECURSOS
650	1	0	_aRECURSOS GENETICOS _9133844
650	1	4	_9140504 _aCIGOTOS
650	1	4	_9141805 _aCONSERVACION BIOLOGICA
650	1	4	_9141719 _aCONGELACION
691			_9140993 _aCOFFEA ARABICA
691			_9164167 _aSEED
691			_9312739 _aCOOLING
691			_9314323 _aDEHYDRATION
691			_9162807 _aRESOURCE CONSERVATION
691			_9149035 _aGENETIC RESOURCES
691			_9343777 _aZYGOTES
691			_9137950 _aBIOLOGICAL PRESERVATION
691			_9319874 _aFREEZING
773	0		_tCryo-Letters (RU) _d1997 _gv. 18 p. 269-276
901			_aF04
902			_aF30
903			_aE
903			_aV
904			_aBCO
905			_aC
906			_a19980101
908			_aJ
942			_cANA _2z