000 038350000a22008530004500
911 _aXL1998502039
901 _aF03
902 _aF30
903 _aE
903 _aV
904 _aBCO
905 _aC
906 _a19980101
908 _aJ
909 _aAS
912 _aEn
912 _aEn
914 _aBCO1
914 _aCAFE
082 0 4 _a633.7314
_bC957c
100 1 _963847
_aDussert, S.
100 1 _956148
_aChabrillange, N.
100 1 _9109
_aEngelmann, F.
100 1 _943095
_aAnthony, F.
100 1 _987745
_aLouarn, J.
100 1 _975866
_aHamon, S.
260 _c1998
041 0 _aEn
500 _a2 tab. 2 fig. 25 ref. Sum. (En)
520 _aIn the range of water contents studied (0.1-0.4 g H2O g dw-1), Coffea arabica seeds were less sensitive to desiccation than C. costatifructa, C. racemosa and C. sessiliflora seeds. At 0.20 g H2O g dw-1, 53 percent of C. arabica seeds germinated after direct immersion in LN (rapid cooling, 200 °C min-1), but none of them developed into normal seedlings. By contrast, in C. costatifructa, C. racemosa and C. sessiliflora, when seeds were dehydrated to the optimal water content (0.19, 0.28 and 0.31 g H2O g dw-1, respectively), the percentages of seeds which developed into normal seedlings after LN exposure were 26, 78 and 31 percent of the desiccation control, respectively. Normal seedlings could be recovered from cryopreserved C. arabica seeds only if they were desiccated to 0.20 g H2O g dw-1 and precooled slowly to -50°C prior to immersion in LN. Precooling seeds at 2°C min-1 allowed 25 percent of seeds to develop into normal seedlings. The thawing rate had no effect on the survival of cryopreserved C. arabica seeds. In all cryopreservation experiments, the total germination did not reflect the percentage of seeds which developed into normal seedlings. Examination of excised embryos indicated a partial explanation of this difference since only the shoot apex was destroyed in abnormal embryos, whereas the hypocotyl and radicle were normal.
650 1 4 _9140993
_aCOFFEA ARABICA
650 1 4 _9141005
_aCOFFEA COSTATIFRUCTA
650 1 4 _9141020
_aCOFFEA RACEMOSA
650 1 4 _9141024
_aCOFFEA SESSILIFLORA
650 1 0 _aSEMILLAS
_9133847
650 1 4 _9168272
_aVIABILIDAD DE LA SEMILLA
650 1 4 _9135409
_aALMACENAMIENTO DE SEMILLAS
650 1 4 _9141719
_aCONGELACION
650 1 4 _9141805
_aCONSERVACION BIOLOGICA
650 1 4 _9142006
_aCONTENIDO DE HUMEDAD
650 1 4 _9145969
_aENFRIAMIENTO
650 1 0 _aRECURSOS GENETICOS
_9133844
650 1 4 _9144137
_aDESHIDRATACION
650 1 4 _98
_aCONSERVACION DE LOS RECURSOS
691 _9140993
_aCOFFEA ARABICA
691 _9141020
_aCOFFEA RACEMOSA
691 _9164167
_aSEED
691 _9337811
_aSEED VIABILITY
691 _9164171
_aSEED STORAGE
691 _9319874
_aFREEZING
691 _9137950
_aBIOLOGICAL PRESERVATION
691 _9327580
_aMOISTURE CONTENT
691 _9312739
_aCOOLING
691 _9149035
_aGENETIC RESOURCES
691 _9314323
_aDEHYDRATION
691 _9162807
_aRESOURCE CONSERVATION
692 _aCOFFEA ARABICA
692 _aCOFFEA RACEMOSA
692 _aSEMENCE
692 _aVIABILITE DES SEMENCES
692 _aSTOCKAGE DES SEMENCES
692 _aCONGELATION
692 _aCONSERVATION BIOLOGIQUE
692 _aTENEUR EN EAU
692 _aREFROIDISSEMENT
692 _aRESSOURCE GENETIQUE
692 _aDESHYDRATATION
692 _aCONSERVATION DES RESSOURCES
773 0 _tSeed Science Research (RU)
_d1998
_gv. 8 p. 9-15
040 _aCR-TuBCO
_cCR-TuBCO
_bEs
245 1 0 _aCryopreservation of seeds of four coffee species (Coffea arabica, C. costatifructa, C. racemosa and C. sessiliflora): importance of water content and cooling rate
942 _cANA
003 CR-TuBCO
999 _c87711
_d87711