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008 210603b1998 ||||| |||| 00| 0 spa d
040 _aCR-TuBCO
_cCR-TuBCO
_bspa
041 0 _aeng
090 _aCATIE
_bL321
100 1 _985048
_aLardet, L.
100 1 _940537
_aAguilar, M.E.
100 1 _994095
_aMichaux-Ferriere, N.
100 _aBerthouly, Marc
_948610
245 1 0 _aEffect of strictly plant-related factors on the response of Hevea brasiliensis and Theobroma cacao nodal explants cultured in vitro
260 _cEne.Mar 1998
300 _b5 figuras y 4 tablas
500 _a
504 _aIncluye 34 referencias bibliográficas.
520 _aNodal cuttings (primary nodal explants) were used to initiate microcutting cultures of Hevea brasiliensis and Theobroma cacao. The physiological stage of growth reached by the branches from which the explants were taken had a considerable influence on culture initiation and response. In both species, the buds of the nodal explants taken from dormant brances (stages 11, 12 and 13 in Theobroma
520 _astage D in Hevea) revealed greater opening ability in vitro and shoot elongation than buds taken from branches in a phase of active meristem growth in Theobroma (stages F1 and F2) or during a foliar growth phase in Hevea (stage C). In Hevea, the branch growth phase effect persisted into the multiplication phase when shoots developed in vitro were used as the source of secondary nodal explants. However, it disappeared during subcultures of primary nodal explants. In both species, the position of the nodes on the branch with respect to the apex also had an effect on culture initiation. The buds of nodes furthest from the apex were more likely to develop into shoots. In Hevea, the position of secondary nodal explants obtained from the primary shoot was seen to vary in effect depending on growth regulator applications during primary culture. These results are analyzed in terms of variations in the balance between endogenous growth promoters/inhibitors in the buds, depending on morphogenic stages and the bud position on the branch.
546 _aResumen en inglés
650 1 4 _9150235
_aHEVEA BRASILIENSIS
650 1 4 _9166664
_aTHEOBROMA CACAO
650 1 4 _9143126
_aCULTIVO IN VITRO
650 1 4 _9147417
_aEXPLANTES
650 1 4 _9142595
_aCRECIMIENTO
650 1 4 _9146697
_aESQUEJES
691 _9150235
_aHEVEA BRASILIENSIS
691 _9166664
_aTHEOBROMA CACAO
691 _9150967
_aIN VITRO CULTURE
691 _9318477
_aEXPLANTS
691 _9149717
_aGROWTH
691 _9313916
_aCUTTINGS
773 0 _tIn vitro Cellular and Development Biology (EUA)
_dEne.Mar 1998
_gv. 34 p. 34-40
901 _aF02
902 _aF62
903 _aE
904 _aBCO
904 _aacarvajal
905 _aC
906 _a20000101
_b20101209
907 _a000003589
907 _a000007713
907 _a000024135
907 _a000027520
907 _a000003394
907 _a000002048
908 _aJ
942 _cANA
_2z