000 026930000a22006010004500
911 _aXL1991005543
901 _aQ04
903 _aE
903 _aV
904 _aBCO
905 _aC
906 _a19910101
908 _aJ
909 _aAS
912 _aEn
912 _aEn
914 _aCACAO
914 _aEXTRA
914 _aBCO1
082 0 4 _a633.7469
_bM149
100 1 _992439
_aMcHenry, L.
100 1 _969274
_aFritz, P.J.
260 _cJul 1987
041 0 _aEn
500 _a4 tab. 8 ref. Sum. (En)
520 _aEnzymes catalyzing the first and last steps in cocoa butter biosynthesis were studied in microsomal preparations from developing cocoa seeds. Both the acyl-CoA:sn-glycerol-3-phosphate 0-acyltransferase (EC 2.3.1.15) and the acyl-CoA:1,2-diacylglycerol 0-acyltransferase (EC 2.3.1.20) showed broad specificity for three fatty acid donors (palmitoyl-CoA, stearoyl-CoA and oleoyl-CoA), but the relative amounts of incorporation of these fatty acids into lysophosphatidic acid and triacylglycerol were influenced by temperature of incubation. The selectivities of the two acyltransferases for saturated vs unsaturated acyl-CoA's also were investigated over a range of temperatures. The data indicate that, in general, the ratio of saturated to unsaturated fatty acids incorporated into glycerol esters increases with increasing temperature of incubation, consistent with the hypothesis that temperature effects on these enzymes contribute to the phenomenon of "soft" cocoa butter, i.e., that cocoa butter obtained from seeds grown in cooler climated has a lower melting point than that prepared from seeds grown in warmer climates.
650 1 4 _9153864
_aMANTECA DE CACAO
650 1 4 _9166664
_aTHEOBROMA CACAO
650 1 4 _9138006
_aBIOSINTESIS
650 1 4 _9167093
_aTRANSFERASAS
650 1 4 _9134288
_aACILTRANSFERASA
650 1 4 _9134279
_aACIDOS GRASOS
650 1 4 _9166359
_aTEMPERATURA
691 _9311335
_aCOCOA BUTTER
691 _9166664
_aTHEOBROMA CACAO
691 _9308012
_aBIOSYNTHESIS
691 _9341371
_aTRANSFERASES
691 _9303979
_aACYLTRANSFERASES
691 _9318782
_aFATTY ACIDS
691 _9166373
_aTEMPERATURE
692 _aBEURRE DE CACAO
692 _aTHEOBROMA CACAO
692 _aBIOSYNTHESE
692 _aTRANSFERASE
692 _aACYLTRANSFERASE
692 _aACIDE GRAS
692 _aTEMPERATURE
773 0 _tJournal of the American Oil Chemists' Society (EUA)
_dJul 1987
_gv. 64(7) p. 1012-1015
040 _aCR-TuBCO
_cCR-TuBCO
_bEs
245 1 0 _aCocoa butter biosynthesis: effect of temperature on Theobroma cacao acyltransferases
942 _cANA
003 CR-TuBCO
999 _c71564
_d71564