000			036550000a22006370004500
911			_aXL1998502030
901			_aF30
902			_aK01
903			_aE
903			_aV
904			_aBCO
905			_aC
906			_a19980101
908			_aJ
909			_aAS
912			_aEn
912			_aEn
914			_aBCO
090			_aAV _b634.97 no. 13
100	1		_971790 _aGillies, A.C.M.
100	1		_959048 _aCornelius, J.P.
100	1		_999140 _aNewton, A.C.
100	1		_998723 _aNavarro, C.
100	1		_977156 _aHernández, M.
100	1		_9131724 _aWilson, J.
260			_c1997
041	0		_aEn
500			_a3 fig. 4 tab. 57 ref. Sum. (En)
520			_aCedrela odorata L. (Spanish cedar), an economically important timber species native to the American tropics, is the focus of increasing conservation concern due to high rates of deforestation within its native range. To assess the extent of the genetic diversity within and between populations of this species, samples were obtained from 10 widely dispersed populations within Costa Rica and analysed for random amplified polymorphic DNA (RAPD) variation. Fourteen 10-mer primers were used to generate 97 polymorphic RAPD bands, Presence/absence data for all bands were subjected to a pairwise genetic distance analysis, according to Jaccard's coefficient, then neighbour-joining cluster analysis was performed on these distances, as was an analysis of molecular variance (AMOVA), to assess levels of differentiation between populations and regions, and Shannon's. Diversity Index was used to quantify levels of diversity within and between populations. Results indicated highly significant genetic differentiation (P<0.004, AMOVA) between populations originating from the North Pacific and Atlantic/South Pacific regions of Costa Rica, with 35.3 percent of the total variation attributable to a difference between these areas. Little differentiation was recorded between populations from within the same region (P=0.757, AMOVA), and 65.1 percent of the total variance was attributable to variation within populations. Estimated values for within-population diversity, calculated as Hpop/Hsp by means of Shannon's Diversity Index, were found to vary greatly between primers, but the overall within-population component of genetic diversity was 0.45. Possible reasons for the high degree of intraspecific genetic variation within this species are discussed and the implications of these results for the conservation and use of its genetic resources are described.
650	1	4	_9139864 _aCEDRELA ODORATA
650	1	4	_9168058 _aVARIACION GENETICA
650	1	4	_9153996 _aMARCADORES GENETICOS
650	1	4	_98 _aCONSERVACION DE LOS RECURSOS
650	1	4	_9136489 _aARBOLES MADERABLES
650	1	0	_aRECURSOS GENETICOS _9133844
650	1	4	_92064 _aCOSTA RICA
691			_9139864 _aCEDRELA ODORATA
691			_9320466 _aGENETIC VARIATION
691			_9149033 _aGENETIC MARKERS
691			_9162807 _aRESOURCE CONSERVATION
691			_9341002 _aTIMBER TREES
691			_9149035 _aGENETIC RESOURCES
691			_aCOSTA RICA _92064
692			_aCEDRELA ODORATA
692			_aVARIATION GENETIQUE
692			_aMARQUEUR GENETIQUE
692			_aCONSERVATION DES RESSOURCES
692			_aARBRE POUR BOIS DE CONSTRUCTION
692			_aRESSOURCE GENETIQUE
692			_aCOSTA RICA
773	0		_tMolecular Ecology (EUA) _d1997 _gv. 6 p. 1133-1145
040			_aCR-TuBCO _cCR-TuBCO _bEs
245	1	0	_aGenetic variation in Costa Rican populations of the tropical timber species Cedrela odorata L., assessed using RAPDs
942			_cANA
003			CR-TuBCO
999			_c107766 _d107766