Cryopreservation on coffee (Coffea arabica L.) seeds: importance of the precooling temperature
Por: Dussert, S
| Chabrillange, N | Engelmann, F | Anthony, F | Hamon, S.
Editor: 1997Tema(s): COFFEA ARABICA | SEMILLAS | ENFRIAMIENTO | DESHIDRATACION | CONSERVACION DE LOS RECURSOS | RECURSOS GENETICOS | CIGOTOS | CONSERVACION BIOLOGICA | CONGELACION | COFFEA ARABICA | SEED | COOLING | DEHYDRATION | RESOURCE CONSERVATION | GENETIC RESOURCES | ZYGOTES | BIOLOGICAL PRESERVATION | FREEZING
En: Cryo-Letters (RU) v. 18 p. 269-276Resumen: The effect of the precooling temperature on the survival rate of cryopreserved Coffea arabica seeds and excised zygotic embryos was investigated. Seeds were desiccated to 0.2 g H2O.g-1 dw, then cooled at 1°C.min-1 to various precooling temperatures. The optimal germination rate (70 percent) of cryopreserved seeds was achieved after precooling to -50°C. After 4 months in culture, 30 percent of seeds developed into normal seedlings. For embryos extracted after thawing from cryopreserved seeds, maximal survival rate (97 percent) was observed when seeds were immersed into liquid nitrogen directly after dehydration, without precooling. All viable embryos developed into normal seedlings after 2 months in culture.
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The effect of the precooling temperature on the survival rate of cryopreserved Coffea arabica seeds and excised zygotic embryos was investigated. Seeds were desiccated to 0.2 g H2O.g-1 dw, then cooled at 1°C.min-1 to various precooling temperatures. The optimal germination rate (70 percent) of cryopreserved seeds was achieved after precooling to -50°C. After 4 months in culture, 30 percent of seeds developed into normal seedlings. For embryos extracted after thawing from cryopreserved seeds, maximal survival rate (97 percent) was observed when seeds were immersed into liquid nitrogen directly after dehydration, without precooling. All viable embryos developed into normal seedlings after 2 months in culture.
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