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Towards the understanding of the cocoa transcriptome: Production and analysis of an exhaustive dataset of ESTs of Theobroma cacao L. generated from various tissues and under various conditions

Por: Argout, X | Fouet, O | Wincker, P | Gramacho, K | Legavre, T | Sabau, X | Risterucci, A.M | Silva, C. Da | Cascardo, J | Phillips, Willard | BioMed Central | BioMed Central, Londres (RU).
Editor: BioMed Central, 2008Descripción: 19 páginas : 7 figuras, 3 tablas.ISSN: 1471-2164.Tema(s): THEOBROMA CACAO | PHYTOPHTHORA PALMIVORA | CERATOCYSTIS FIMBRIATA | MONILIOPHTHORA | TERPENOIDOS | CLONES | GENOTIPOS | MARCADORES GENETICOS | RESISTENCIA A AGENTES DANINOS | RESISTENCIA A LA ENFERMEDAD | INOCULACION | FITOMEJORAMIENTO | CALIDAD | SABOR | THEOBROMA CACAO | PHYTOPHTHORA PALMIVORA | CERATOCYSTIS FIMBRIATA | MONILIOPHTHORA | TERPENOIDS | CLONES | GENOTYPES | GENETIC MARKERS | RESISTANCE TO INJURIOUS FACTORS | DISEASE RESISTANCE | INOCULATION | PLANT BREEDING | QUALITY | FLAVOURRecursos en línea: eng En: BMC Genomics Volumen 9, número 512, páginas 1-19Resumen: Theobroma cacao L., is a tree originated from the tropical rainforest of South America. It is one of the major cash crops for many tropical countries. T. cacao is mainly produced on smallholdings, providing resources for 14 million farmers. Disease resistance and T. cacao quality improvement are two important challenges for all actors of cocoa and chocolate production. T. cacao is seriously affected by pests and fungal diseases, responsible for more than 40% yield losses and quality improvement, nutritional and organoleptic, is also important for consumers. An international collaboration was formed to develop an EST genomic resource database for cacao.Fifty-six cDNA libraries were constructed from different organs, different genotypes and different environmental conditions. A total of 149,650 valid EST sequences were generated corresponding to 48,594 unigenes, 12,692 contigs and 35,902 singletons. A total of 29,849 unigenes shared significant homology with public sequences from other species.

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Theobroma cacao L., is a tree originated from the tropical rainforest of South America. It is one of the major cash crops for many tropical countries. T. cacao is mainly produced on smallholdings, providing resources for 14 million farmers. Disease resistance and T. cacao quality improvement are two important challenges for all actors of cocoa and chocolate production. T. cacao is seriously affected by pests and fungal diseases, responsible for more than 40% yield losses and quality improvement, nutritional and organoleptic, is also important for consumers. An international collaboration was formed to develop an EST genomic resource database for cacao.Fifty-six cDNA libraries were constructed from different organs, different genotypes and different environmental conditions. A total of 149,650 valid EST sequences were generated corresponding to 48,594 unigenes, 12,692 contigs and 35,902 singletons. A total of 29,849 unigenes shared significant homology with public sequences from other species.

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