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Métodos de inoculación para la producción in vivo de Bacillus popilliae en larvas de Phyllophaga menetriesi

Por: Hidalgo, E | Flores, L | CATIE, Turrialba (Costa Rica). Programa de Investigación | 4. Semana Científica Turrialba (Costa Rica) 6-9 Abr 1999.
Series Serie Técnica. Reuniones Técnicas (CATIE).Editor: Turrialba, Costa Rica 1999Descripción: p. 104-107.ISBN: 9977573190.Tema(s): BACILLUS POPILLIAE | PHYLLOPHAGA MENETRIESI | INOCULACION | LARVAS | INYECCION | BACILLUS POPILLIAE | INOCULATION | LARVAE | INJECTIONRecursos en línea: Haga clic para acceso en línea En: En: CATIE, Turrialba (Costa Rica). Programa de Investigación Logros de la investigación para el nuevo milenio. ActasResumen: A laboratory experiment was carried out to compare three inoculation methods of spore suspensions of two Bacillus popilliae strains. Third instar Phyllophaga menetriesi larvae were inoculated with a dosel of 2.5x10 exponente 6 Bp spores/larva by injection, topical application and fed with inoculated artificial diet. The highest infection was obtained in the injected treatment with the strain 524 (67 percent), follow by the strain 504 in the artificial diet treatment with 50 percent infection. A high percentage of asymptomatic mortality was observed with the strain 504 when topically inoculated.

1 fig. 6 ref. Sum. (En)

A laboratory experiment was carried out to compare three inoculation methods of spore suspensions of two Bacillus popilliae strains. Third instar Phyllophaga menetriesi larvae were inoculated with a dosel of 2.5x10 exponente 6 Bp spores/larva by injection, topical application and fed with inoculated artificial diet. The highest infection was obtained in the injected treatment with the strain 524 (67 percent), follow by the strain 504 in the artificial diet treatment with 50 percent infection. A high percentage of asymptomatic mortality was observed with the strain 504 when topically inoculated.

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